This items is made to order and is not returnable and order cannot be canceled.

CUSTOM ITEM: Guard Column Starter Set, Bidentate C8, complete with 5 each 4.0 mm ID x 10 mm long cartridges packed with 5 um, 300 A phase and 1 each guard column holder. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 40008‑PG4‑3 Cogent Bidentate C8 Guard Column Starter Set is a complete protection system for standard‑flow analytical HPLC columns, specifically optimized for wide‑pore (300 Å) separations involving biomolecules and larger analytes.

This starter kit includes:

Each cartridge is individually stored in a closeable Delrin™ case, protecting against contamination, drying, and mechanical damage prior to use.

The included holder is compatible with all Cogent TYPE‑C™ guard cartridges, allowing flexibility across multiple methods and phases.

Designed for Standard Analytical Column IDs

This starter kit is optimized for:

  • 3.0 mm ID solvent‑saving columns

  • 4.0 mm ID analytical columns

  • 4.6 mm ID standard HPLC columns

This makes it ideal for conventional HPLC systems requiring reliable protection across a range of flow rates and methods.

Proper guard column selection ensures:

  • Minimal band broadening

  • Maintained separation efficiency

  • Consistent chromatographic performance

Why Use a 300 Å Guard Column System

The 300 Å pore size is designed for:

  • Peptides and proteins

  • Large molecular weight compounds

  • Complex biological and matrix‑heavy samples

Compared to 100 Å phases, 300 Å:

  • Improves accessibility for larger molecules

  • Reduces steric exclusion effects

  • Enhances recovery and reproducibility in biomolecule analysis

Why Use a Guard Column Starter Kit

Analytical columns are exposed to:

  • Particulates and debris

  • Strongly retained contaminants

  • Sample matrix buildup

  • Irreversible fouling

The 40008‑PG4‑3 starter kit provides a sacrificial protection layer, helping to:

  • Extend analytical column lifetime significantly

  • Maintain reliable chromatographic performance

  • Reduce replacement frequency and cost

  • Improve reproducibility across runs

Under typical laboratory conditions, the included five cartridges often provide sufficient protection for the lifetime of an analytical column, depending on sample cleanliness.

Bidentate C8 Guard Phase Advantages

Matching the guard column phase to your analytical column ensures:

  • Consistent selectivity and retention behavior

  • No disruption to method performance

  • Seamless analyte transfer

Compared to C18 phases, Bidentate C8:

  • Reduces excessive hydrophobic retention

  • Improves recovery of amine‑containing compounds

  • Provides alternative selectivity for complex separations

  • Supports faster equilibration cycles

Cogent TYPE‑C™ Technology Benefits

Silica Hydride Surface

  • Reduced silanol activity

  • Improved peak shape for basic and polar compounds

  • Lower secondary interactions

Faster Equilibration

  • Reduced cycle time between injections

  • Increased throughput in analytical workflows

Durability

  • Resistant to hydrolysis

  • Long cartridge and column life

  • Stable under demanding analytical conditions

Typical Applications

  • LC‑MS and HPLC biomolecule analysis

  • Peptides and protein separations

  • Pharmaceutical and bioanalytical workflows

  • Complex biological sample matrices

  • Methods requiring wide‑pore stationary phases

Made‑to‑Order & Pricing Information

This is a custom, made‑to‑order item.

  • Orders cannot be canceled once placed

  • Custom items are non‑returnable

Custom Order Item. Inquire for more information.

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